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KMID : 1098420050130030081
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Korean Journal of Medicinal Crop Science
2005 Volume.13 No. 3 p.81 ~ p.86
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Enhancement of Immune Activities of Ephedrae Herba and Rubi Fructus at Low Temperature Extraction
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Kim Dae-Ho
Park JIn-Hong
Kim Jung-Hwa
Kim Cheol-Hee
You Jin-Hyun
Kwon Min-Cheol
Lee Hyeon-Yong
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Abstract
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The immune activities of the extracts from Ephedrae Herba and Rubi Frutus extraction with ultrasonification at 60^{circ}C were compared with the extracts though water extraction at 100^{circ}C. The growth of human T cells was increased up to 13{times}10^4;viable;cells/m{ell} in adding 1.0;g/{ell} of the ultrasonification extracts of R. Fructus at 60^{circ}C, compared to adding the extracts at 100^{circ}C. The secretion of TNF-{alpha} for human T cells were also increased up to 13.9{times}10^{-4} pg/cell by adding extracts at 60^{circ}C, compared to extracts at 100^{circ}C. The extracts of R. Fructus at 60^{circ}C increased NK cell activities up to 50% and the secretion of NO^{-1} from macrophage to 31;{uu}M for ultrasonification extracts at 60^{circ}C.
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KEYWORD
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ultrasonification, Ephedrae Herba, Rubi Fructus, human B and T cells, NK cell
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